Human HLA-F Recombinant Protein (RPPB3693)
- SKU:
- RPPB3693
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P30511
Description
| Product Name: | Human HLA-F Recombinant Protein |
| Product Code: | RPPB3693 |
| Size: | 10µg |
| Species: | Human |
| Target: | HLA-F |
| Synonyms: | CDA12, HLA-5.4, HLA-CDA12, HLAF, HLA class I histocompatibility antigen, alpha chain F, HLA F antigen, Leukocyte antigen F, MHC class I antigen F, HLA-F. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | HLA-F protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSGSHSLRY FSTAVSRPGR GEPRYIAVEY VDDTQFLRFD SDAAIPRMEP REPWVEQEGP QYWEWTTGYA KANAQTDRVA LRNLLRRYNQ SEAGSHTLQG MNGCDMGPDG RLLRGYHQHA YDGKDYISLN EDLRSWTAAD TVAQITQRFY EAEEYAEEFR TYLEGECLEL LRRYLENGKE TLQRADPPKA HVAHHPISDH EATLRCWALG FYPAEITLTW QRDGEEQTQD TELVETRPAG DGTFQKWAAV VVPPGEEQRY TCHVQHEGLP QPLILRWEQS PQPTIPI |
Major Histocompatibility Complex Class I F (HLA-F) belongs to the MHC family which takes part in the presentation of antigens to the T cell receptor. HLA-F is a member of the class I molecules which are expressed in virtually all cells. There are 2 classes of HLA antigens. Class I molecules takes an important part in the immune system by presenting peptides derived from the endoplasmic reticulum.
HLA-F Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 307 amino acids (22-305a.a) and having a molecular mass of 35.1kDa. HLA-F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | HLA-F: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: Golgi membrane; phagocytic vesicle membrane; cell surface; membrane; endoplasmic reticulum; early endosome membrane; plasma membrane; MHC class I protein complex Molecular Function:TAP1 binding; peptide antigen binding; TAP2 binding; receptor binding Biological Process: regulation of immune response; antigen processing and presentation of peptide antigen via MHC class I; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I |
| NCBI Summary: | This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P30511 |
| NCBI GenInfo Identifier: | 149158700 |
| NCBI Gene ID: | 3134 |
| NCBI Accession: | NP_001091948.1 |
| UniProt Secondary Accession: | P30511,Q5JQI8, Q5JQJ1, Q5SPT5, Q860R0, Q8MGQ1, Q8WLP5 Q95HC0, Q9TP68, |
| UniProt Related Accession: | P30511 |
| Molecular Weight: | 50,438 Da |
| NCBI Full Name: | HLA class I histocompatibility antigen, alpha chain F isoform 3 |
| NCBI Synonym Full Names: | major histocompatibility complex, class I, F |
| NCBI Official Symbol: | HLA-F�� |
| NCBI Official Synonym Symbols: | HLAF; CDA12; HLA-5.4; HLA-CDA12�� |
| NCBI Protein Information: | HLA class I histocompatibility antigen, alpha chain F |
| UniProt Protein Name: | HLA class I histocompatibility antigen, alpha chain F |
| UniProt Synonym Protein Names: | CDA12; HLA F antigen; Leukocyte antigen F; MHC class I antigen F |
| Protein Family: | HLA class I histocompatibility antigen |
| UniProt Gene Name: | HLA-F�� |
| UniProt Entry Name: | HLAF_HUMAN |
