The Human HS (Heparan Sulfate) ELISA Kit is a highly reliable and sensitive tool for the precise detection of heparan sulfate levels in human samples including serum, plasma, and cell culture supernatants. With its high specificity and accuracy, this kit provides consistent and reproducible results, making it ideal for a variety of research purposes.Heparan sulfate is a critical component of the extracellular matrix, playing a key role in various physiological and pathological processes including cell signaling, growth factor binding, and development. Abnormalities in heparan sulfate levels have been linked to diseases such as cancer, diabetes, and inflammatory disorders, highlighting its importance as a biomarker for disease diagnosis and therapeutic development.With the Human HS ELISA Kit, researchers can confidently study the role of heparan sulfate in disease progression and explore potential treatment strategies with confidence and precision.
Product Name:
Human HS (heparan sulfate) ELISA Kit
SKU:
HUES03235
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
