The Human HSP-40 (Heat Shock Protein 40) CLIA Kit is specifically designed for the precise measurement of HSP-40 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, which makes it an invaluable tool for various research purposes.HSP-40 is a critical chaperone protein that assists in protein folding and plays a key role in cellular stress response. Dysregulation of HSP-40 has been linked to various diseases including cancer, neurodegenerative disorders, and cardiovascular conditions.
Therefore, studying HSP-40 levels can provide valuable insights into the pathogenesis of these diseases and aid in the development of potential therapeutic interventions.Overall, the Human HSP-40 CLIA Kit offers researchers a reliable and efficient means of assessing HSP-40 levels, allowing for in-depth investigation into the mechanisms underlying various diseases and facilitating the discovery of novel treatment strategies.
Product Name:
Human HSP-40 (Heat Shock Protein 40) CLIA Kit
SKU:
HUES01028
Target:
Human HSP-40 (Heat Shock Protein 40)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-40. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-40, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HSP-40. You can calculate the concentration of Human HSP-40 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-117
93-106
92-106
Average (%)
108
98
97
1:4
Range (%)
92-109
91-102
101-119
Average (%)
100
96
109
1:8
Range (%)
97-111
103-118
94-105
Average (%)
103
109
100
1:16
Range (%)
97-115
95-107
90-100
Average (%)
105
102
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
100
EDTA plasma (n=5)
85-99
92
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.1
176.93
877.45
99.93
193.57
797.78
Standard deviation
9.47
14.9
83.8
12.28
23.17
48.51
C V (%)
9.1
8.42
9.55
12.29
11.97
6.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HSP-40 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HSP-40 in samples. No significant cross-reactivity or interference between Human HSP-40 and analogues was observed.
