Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit
The Human Intercellular Adhesion Molecule 1 (ICAM-1/CD54) ELISA Kit is a cutting-edge assay designed for the precise quantitative detection of ICAM-1 levels in various human biological samples. ICAM-1, a cell surface glycoprotein, plays a crucial role in mediating adhesion processes and immune responses, particularly during inflammation. Elevated ICAM-1 expression is associated with various inflammatory and immune-related conditions, making accurate measurement vital for understanding disease mechanisms and exploring potential therapeutic interventions. Benefit from Assay Genie's ICAM-1 ELISA Kit, known for its exceptional sensitivity and specificity, ensuring reproducible and dependable results. Manufactured under rigorous quality control standards, this kit offers robust performance and user-friendly protocols, making it an ideal choice for sensitive and specific detection of ICAM-1 biomarker levels in research studies. Trust Assay Genie's ICAM-1 ELISA Kit for accurate quantification and insightful analysis in your scientific investigations.
Product Name:
Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit
SKU:
AEES00145
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
