Human IDE Recombinant Protein (RPPB1838)
- SKU:
- RPPB1838
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P14735
- Research Area:
- Enzymes
Description
| Product Name: | Human IDE Recombinant Protein |
| Product Code: | RPPB1838 |
| Size: | 10µg |
| Species: | Human |
| Target: | IDE |
| Synonyms: | Insulin-Degrading Enzyme, Abeta-Degrading Protease, Insulin Protease, EC 3.4.24.56, Insulinase, INSULYSIN, Insulysin, EC 3.4.24, IDE. |
| Source: | Escherichia Coli |
| Physical Appearance: | Filtered colorless solution. |
| Formulation: | IDE filtered (0.4�m) solution at a concentration of 0.6mg/ml in 20mM Tris buffer, 50mM NaCl, pH 8.0 and 10% (w/v) glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRYRLAWLLH PALPSTFRSV LGARLPPPER LCGFQKKTYS KMNNPAIKRI GNHITKSPED KREYRGLELA NGIKVLLISD PTTDKSSAAL DVHIGSLSDP PNIAGLSHFC EHMLFLGTKK YPKENEYSQF LSEHAGSSNA FTSGEHTNYY FDVSHEHLEG ALDRFAQFFL CPLFDESCKD REVNAVDSEH EKNVMNDAWR LFQLEKATGN PKHPFSKFGT GNKYTLETRP NQEGIDVRQE LLKFHSAYYS SNLMAVCVLG RESLDDLTNL VVKLFSEVEN KNVPLPEFPE HPFQEEHLKQ LYKIVPIKDI RNLYVTFPIP DLQKYYKSNP GHYLGHLIGH EGPGSLLSEL KSKGWVNTLV GGQKEGARGF MFFIINVDLT EEGLLHVEDI ILHMFQYIQK LRAEGPQEWV FQECKDLNAV AFRFKDKERP RGYTSKIAGI LHYYPLEEVL TAEYLLEEFR PDLIEMVLDK LRPENVRVAI VSKSFEGKTD RTEEWYGTQY KQEAIPDEVI KKWQNADLNG KFKLPTKNEF IPTNFEILPL EKEATPYPAL IKDTAMSKLW FKQDDKFFLP KACLNFEFFS PFAYVDPLHC NMAYLYLELL KDSLNEYAYA AELAGLSYDL QNTIYGMYLS VKGYNDKQPI LLKKIIEKMA TFEIDEKRFE IIKEAYMRSL NNFRAEQPHQ HAMYYLRLLM TEVAWTKDEL KEALDDVTLP RLKAFIPQLL SRLHIEALLH GNITKQAALG IMQMVEDTLI EHAHTKPLLP SQLVRYREVQ LPDRGWFVYQ QRNEVHNNCG IEIYYQTDMQ STSENMFLEL FCQIISEPCF NTLRTKEQLG YIVFSGPRRA NGIQGLRFII QSEKPPHYLE SRVEAFLITM EKSIEDMTEE AFQKHIQALA IRRLDKPKKL SAECAKYWGE IISQQYNFDR DNTEVAYLKT LTKEDIIKFY KEMLAVDAPR RHKVSVHVLA REMDSCPVVG EFPCQNDINL SQAPALPQPE VIQNMTEFKR GLPLFPLVKP HINFMAAKL E HHHHHH |
Insulin-Degrading Enzyme (IDE) is a zinc metallopeptidase which degrades intracellular insulin, and thus terminates insulins activity, as well as playing a part in intercellular peptide signaling by degrading various peptides such as amylin, bradykinin, and kallidin. The preferential affinity of the IDE enzyme for insulin results in insulin-mediated inhibition of the degradation of additional peptides such as beta-amyloid. Deficiencies in IDE protein's function are linked with Alzheimer's disease and type 2 diabetes mellitus nevertheless mutations in the IDE gene have not been demonstrated to be causative for these diseases. Insulin-Degrading Enzyme localizes mainly to the cytoplasm however in some cell types it localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. In addition, IDE degrades amyloid formed by APP and IAPP. Furthermore, IDE plays a part in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
IDE Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Met1-Leu1019) containing 1026 amino acids including a 7 aa His tag at C-terminus. The total calculated molecular mass is 119kDa.
| UniProt Protein Function: | IDE: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. Belongs to the peptidase M16 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell surface; EC 3.4.24.56; Nuclear receptor co-regulator; Protease Chromosomal Location of Human Ortholog: 10q23.33 Cellular Component: cell surface; cytoplasm; cytosol; extracellular space; mitochondrion; nucleus; peroxisome Molecular Function:ATP binding; glycoprotein binding; insulin binding; metalloendopeptidase activity; peptide binding; protein binding; protein homodimerization activity; receptor binding; ubiquitin binding; zinc ion binding Biological Process: beta-amyloid metabolic process; determination of adult life span; positive regulation of protein oligomerization; protein homooligomerization; proteolysis; proteolysis involved in cellular protein catabolic process |
| NCBI Summary: | This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009] |
| UniProt Code: | P14735 |
| NCBI GenInfo Identifier: | 215274252 |
| NCBI Gene ID: | 3416 |
| NCBI Accession: | P14735.4 |
| UniProt Secondary Accession: | P14735,Q5T5N2, B2R721, B7ZAU2, D3DR35, |
| UniProt Related Accession: | P14735 |
| Molecular Weight: | 118kDa |
| NCBI Full Name: | Insulin-degrading enzyme |
| NCBI Synonym Full Names: | insulin degrading enzyme |
| NCBI Official Symbol: | IDE�� |
| NCBI Official Synonym Symbols: | INSULYSIN�� |
| NCBI Protein Information: | insulin-degrading enzyme |
| UniProt Protein Name: | Insulin-degrading enzyme |
| UniProt Synonym Protein Names: | Abeta-degrading protease; Insulin protease; Insulinase; Insulysin |
| Protein Family: | IgM protease |
| UniProt Gene Name: | IDE�� |
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