Human IFI30 Recombinant Protein (RPPB0377)
- SKU:
- RPPB0377
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P13284
- Research Area:
- Cytokines
Description
| Product Name: | Human IFI30 Recombinant Protein |
| Product Code: | RPPB0377 |
| Size: | 10µg |
| Species: | Human |
| Target: | IFI30 |
| Synonyms: | IFI30, Gamma-IFN-Inducible Lysosomal Thiol Reductase, IFN Gamma-Inducible Protein 30 Preproprotein, Gamma-IFN-Inducible Protein IP-30, Legumaturain, GILT, IP30, IFI-30, MGC32056, EC 1.8. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The IFI30 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMNAPLVN VTLYYEALCG GCRAFLIREL FPTWLLVMEI LNVTLVPYGN AQEQNVSGRW EFKCQHGEEE CKFNKVEACV LDELDMELAF LTIVCMEEFE DMERSLPLCL QLYAPGLSPD TIMECAMGDR GMQLMHANAQ RTDALQPPHE YVPWVTVNGK� PLEDQTQLLT LVCQLYQGK |
IFNI30 inducible lysosomal thiol reductase (IFI30), is a part of the GILT family. IFI30 is a lysosomal thiol reductase which at low pH is capable of decreasing protein�s disulfide bonds. IFI30 is expressed constitutively in antigen-presenting cells and induced by gamma-IFN in other cell types. Also, IFI30 plays an important role in MHC class II-restricted antigen processing. IFI30 facilitates the generation of MHC class II-restricted epitopes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds and Also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or cross-presentation.
IFI30 Human Recombinant produced in E. coli is a single polypeptide chain containing 199 amino acids (58-232) and having a molecular mass of 22.5 kDa. IFI30 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IFI30: Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II- restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation. Belongs to the GILT family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; EC 1.8.-.-; Secreted Chromosomal Location of Human Ortholog: 19p13.1 Cellular Component: lysosomal lumen; intracellular membrane-bound organelle; lysosome; cytoplasm; extracellular region; plasma membrane; cell junction Molecular Function:oxidoreductase activity, acting on sulfur group of donors Biological Process: protein stabilization; negative regulation of fibroblast proliferation; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class II |
| NCBI Summary: | The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P13284 |
| NCBI GenInfo Identifier: | 29150261 |
| NCBI Gene ID: | 10437 |
| NCBI Accession: | NP_006323.2 |
| UniProt Secondary Accession: | P13284,Q76MF9, Q8NEI4, Q8WU77, Q9UL08, |
| UniProt Related Accession: | P13284 |
| Molecular Weight: | 27,964 Da |
| NCBI Full Name: | gamma-interferon-inducible lysosomal thiol reductase preproprotein |
| NCBI Synonym Full Names: | interferon, gamma-inducible protein 30 |
| NCBI Official Symbol: | IFI30�� |
| NCBI Official Synonym Symbols: | GILT; IP30; IP-30; IFI-30�� |
| NCBI Protein Information: | gamma-interferon-inducible lysosomal thiol reductase; gamma-interferon-inducible protein IP-30; interferon gamma-inducible protein 30 preproprotein; legumaturain |
| UniProt Protein Name: | Gamma-interferon-inducible lysosomal thiol reductase |
| UniProt Synonym Protein Names: | Gamma-interferon-inducible protein IP-30; Legumaturain |
| Protein Family: | GILT-like protein |
| UniProt Gene Name: | IFI30�� |
| UniProt Entry Name: | GILT_HUMAN |
