The Human IFN-Gamma (Interferon-Gamma) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of IFN-Gamma levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an essential tool for researchers studying immune responses and inflammatory diseases.IFN-Gamma is a key cytokine involved in the immune response, playing a crucial role in the activation of macrophages, promoting antigen presentation, and enhancing the cytotoxicity of T cells.
Dysregulation of IFN-Gamma levels has been implicated in various autoimmune disorders, infectious diseases, and cancer, highlighting its importance as a biomarker for disease monitoring and therapeutic development.With its user-friendly format and accurate performance, the Human IFN-Gamma CLIA Kit from Assay Genie provides researchers with a valuable tool for investigating the role of IFN-Gamma in health and disease.
Product Name:
Human IFN-gamma (Interferon Gamma) CLIA Kit
SKU:
HUES00104
Target:
Human IFN-gamma (Interferon Gamma)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-γ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IFN-γ. You can calculate the concentration of Human IFN-γ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
103-116
86-100
Average (%)
94
110
91
1:4
Range (%)
96-108
93-107
102-116
Average (%)
103
99
107
1:8
Range (%)
86-98
93-108
92-107
Average (%)
91
100
98
1:16
Range (%)
101-111
101-116
89-105
Average (%)
106
107
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
105
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
92-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.04
95.24
281.15
37.88
95.49
283.71
Standard deviation
3.0
10.67
26.29
3.62
7.63
24.51
C V (%)
8.1
11.2
9.35
9.56
7.99
8.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IFN-γ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IFN-γ in samples. No significant cross-reactivity or interference between Human IFN-γ and analogues was observed.
