The Human IFNα (Interferon Alpha) ELISA Kit is expertly designed to facilitate the quantitative measurement of Interferon Alpha in diverse human biological samples. Interferon Alpha plays a vital role in the body's immune response, particularly in combating viral infections and regulating immune cell activities. It serves as a critical cytokine with implications in antiviral defense, immune modulation, and overall immune system function. Our ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results essential for elucidating the role of Interferon Alpha in immune responses and immune-related disorders.
With stringent quality control measures in place, this kit delivers reliable performance and user-friendly protocols, making it an invaluable tool for research applications. Trust in Assay Genie's IFNα ELISA Kit for dependable quantification and thorough exploration of this key biomarker in your research studies.
Product Name:
Human IFNalpha (Interferon Alpha) ELISA Kit
SKU:
AEES00153
Target:
Human IFNα (Interferon Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFNα. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFNα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFNα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFNα. You can calculate the concentration of Human IFNα in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
97-112
92-109
Average (%)
94
104
99
1:4
Range (%)
88-101
99-113
93-109
Average (%)
95
107
100
1:8
Range (%)
87-98
93-106
92-107
Average (%)
92
99
100
1:16
Range (%)
84-95
96-108
90-106
Average (%)
90
102
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
91-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.29
65.91
237.77
21.2
60.1
221.45
Standard deviation
1.49
3.41
10.94
1.19
3.05
11.03
C V (%)
6.39
5.17
4.6
5.6
5.07
4.98
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IFNα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IFNα in samples. No significant cross-reactivity or interference between Human IFNα and analogues was observed.
