The Human IgE (Immunoglobulin E) ELISA Kit is specifically designed for the precise quantitative detection of IgE levels in various human biological samples. IgE is a key immunoglobulin involved in allergic responses, hypersensitivity reactions, and immune defense against parasites. Accurate measurement of IgE levels is crucial for studying allergic diseases, asthma, and other immunological disorders where IgE plays a significant role in disease pathology.
Our IgE ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research needs. Manufactured under stringent quality control standards, this kit delivers robust performance and ease of use, making it an excellent choice for research applications. Trust Assay Genie's IgE ELISA Kit for dependable quantification of this important immunoglobulin in your studies.
Product Name:
Human IgE (Immunoglobulin E) ELISA Kit
SKU:
AEES00138
Target:
Human IgE (Immunoglobulin E)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IgE. You can calculate the concentration of Human IgE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
85-100
89-99
Average (%)
96
93
96
1:4
Range (%)
85-100
91-102
86-101
Average (%)
93
96
93
1:8
Range (%)
89-97
88-102
96-108
Average (%)
92
95
101
1:16
Range (%)
86-98
90-100
93-105
Average (%)
93
95
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-100
92
EDTA plasma (n=5)
90-103
97
Cell culture media (n=5)
98-107
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
8.84
22.74
86.16
9.33
24.24
94.98
Standard deviation
0.48
1.06
3.66
0.55
1.13
4.69
C V (%)
5.43
4.66
4.25
5.89
4.66
4.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IgE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IgE in samples. No significant cross-reactivity or interference between Human IgE and analogues was observed.
