Human IGFBP-1 (Insulin Like Growth Factor Binding Protein 1) ELISA Kit
The Human IGFBP-1 (Insulin-Like Growth Factor Binding Protein-1) ELISA Kit is a reliable tool for the precise measurement of IGFBP-1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.IGFBP-1 is a key regulator of insulin-like growth factor (IGF) signaling, playing a crucial role in modulating cell growth, proliferation, and survival. Dysregulation of IGFBP-1 has been linked to various diseases such as diabetes, cancer, and metabolic disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions. By using the Human IGFBP-1 ELISA Kit, researchers can gain valuable insights into the role of IGFBP-1 in disease pathogenesis and progression, ultimately advancing our understanding of the molecular mechanisms underlying these complex conditions. This kit is a valuable resource for studies aiming to elucidate the intricate interplay between IGF signaling and disease development.
Product Name:
Human IGFBP-1 (Insulin Like Growth Factor Binding Protein 1) ELISA Kit
SKU:
HUES01654
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
