Human IGFBP-5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit
The Human IGFBP-5 (Insulin-like Growth Factor Binding Protein 5) ELISA Kit is specifically designed for the precise measurement of IGFBP-5 levels in human biological samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results that are ideal for a variety of research applications.IGFBP-5 is a key protein involved in regulating the activity of insulin-like growth factors, playing a crucial role in cell growth, differentiation, and survival.
Abnormal levels of IGFBP-5 have been associated with various diseases such as cancer, diabetes, and osteoporosis, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.For researchers seeking reliable and high-quality tools to investigate the role of IGFBP-5 in human health and disease, the Human IGFBP-5 ELISA Kit from Assay Genie is an essential addition to the laboratory toolkit.
Product Name:
Human IGFBP-5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit
SKU:
HUES01655
Target:
Human IGFBP-5 (Insulin Like Growth Factor Binding Protein 5)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGFBP-5. You can calculate the concentration of Human IGFBP-5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
95-110
95-109
Average (%)
96
102
102
1:4
Range (%)
87-97
81-90
86-98
Average (%)
92
86
92
1:8
Range (%)
91-106
86-100
88-103
Average (%)
98
92
94
1:16
Range (%)
95-107
86-100
83-95
Average (%)
100
93
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
90-103
98
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.92
13.02
38.81
5.29
14.25
39.13
Standard deviation
0.26
0.53
2.02
0.34
0.83
2.11
C V (%)
5.28
4.07
5.2
6.43
5.82
5.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IGFBP-5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGFBP-5 in samples. No significant cross-reactivity or interference between Human IGFBP-5 and analogues was observed.
