The Human IgG (Immunoglobulin G) ELISA Kit is specifically designed for the quantitative detection of IgG levels in human serum and plasma samples. This kit provides accurate and reliable results with high sensitivity and specificity, making it an ideal tool for research in immunology, infectious diseases, and autoimmune disorders. Immunoglobulin G is the most abundant antibody in the human body and plays a crucial role in immune response by recognizing and binding to pathogens. Monitoring IgG levels can provide valuable insights into the immune status of an individual, making this kit a valuable resource for studying immune system function and response to various pathogens. With its user-friendly protocol and efficient performance, the Human IgG ELISA Kit from Assay Genie is an essential tool for researchers and clinicians looking to investigate immune responses and develop new diagnostic and therapeutic strategies.
Product Name:
Human IgG (Immunoglobulin G) ELISA Kit
SKU:
HUES01439
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
