The Human IgG1 (Immunoglobulin G1) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of levels of IgG1 in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.IgG1 is a major class of immunoglobulins involved in the immune response, playing a key role in antibody-mediated immunity.
Elevated levels of IgG1 have been associated with various autoimmune diseases, allergies, and infectious diseases, making it a valuable biomarker for studying and diagnosing these conditions.The Human IgG1 CLIA Kit from Assay Genie offers researchers a reliable and efficient tool for measuring IgG1 levels in biological samples, providing valuable insights into the immune response and potential therapeutic targets.
Product Name:
Human IgG1 (Immunoglobulin G1) CLIA Kit
SKU:
HUES00972
Target:
Human IgG1 (Immunoglobulin G1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 μg/mL
Detection range:
0.31-20 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IgG1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgG1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IgG1. You can calculate the concentration of Human IgG1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-117
86-101
100-115
Average (%)
109
92
108
1:4
Range (%)
89-102
85-96
94-110
Average (%)
96
91
101
1:8
Range (%)
98-112
96-110
98-109
Average (%)
106
101
104
1:16
Range (%)
90-105
89-104
95-109
Average (%)
96
96
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
105
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
90-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.06
2.28
9.51
1.08
2.25
9.19
Standard deviation
0.13
0.19
0.74
0.1
0.21
0.85
C V (%)
12.26
8.33
7.78
9.26
9.33
9.25
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IgG1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IgG1 in samples. No significant cross-reactivity or interference between Human IgG1 and analogues was observed.
