The Human IgG3 (Immunoglobulin G3) ELISA Kit is meticulously crafted for the quantitative determination of Immunoglobulin G3 levels in various human sample types. Immunoglobulin G3 is a subclass of the IgG antibody that plays a pivotal role in the humoral immune response, particularly in defense against pathogens. Accurate measurement of IgG3 is paramount for understanding immune responses, infectious diseases, and autoimmune conditions, making it a valuable target for research and clinical studies.
Our ELISA kit ensures exceptional sensitivity and specificity, delivering precise and reproducible results in the quantification of IgG3 levels. Manufactured under strict quality control measures, the kit offers robust performance and user-friendly protocols, catering to both research and clinical applications. Rely on Assay Genie's Human IgG3 ELISA Kit for dependable and accurate quantification of this essential immunoglobulin subclass in your experiments.
Product Name:
Human IgG3 (Immunoglobulin G3) ELISA Kit
SKU:
AEES00123
Target:
Human IgG3 (Immunoglobulin G3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgG3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgG3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgG3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IgG3. You can calculate the concentration of Human IgG3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
88-97
89-103
Average (%)
91
91
95
1:4
Range (%)
93-102
97-110
91-102
Average (%)
97
104
98
1:8
Range (%)
90-98
91-104
87-98
Average (%)
94
97
95
1:16
Range (%)
100-109
99-106
95-106
Average (%)
105
102
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
104
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
84-95
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
86.07
224.44
984.91
94.65
249.69
807.85
Standard deviation
4.61
11.0
48.75
4.98
12.48
44.35
C V (%)
5.36
4.9
4.95
5.26
5.0
5.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IgG3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IgG3 in samples. No significant cross-reactivity or interference between Human IgG3 and analogues was observed.
