The Human IgG3 (Immunoglobulin G3) ELISA Kit is meticulously crafted for the quantitative determination of Immunoglobulin G3 levels in various human sample types. Immunoglobulin G3 is a subclass of the IgG antibody that plays a pivotal role in the humoral immune response, particularly in defense against pathogens. Accurate measurement of IgG3 is paramount for understanding immune responses, infectious diseases, and autoimmune conditions, making it a valuable target for research and clinical studies. Our ELISA kit ensures exceptional sensitivity and specificity, delivering precise and reproducible results in the quantification of IgG3 levels. Manufactured under strict quality control measures, the kit offers robust performance and user-friendly protocols, catering to both research and clinical applications. Rely on Assay Genie's Human IgG3 ELISA Kit for dependable and accurate quantification of this essential immunoglobulin subclass in your experiments.
Product Name:
Human IgG3 (Immunoglobulin G3) ELISA Kit
SKU:
AEES00123
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
