The Human IL-1 Alpha ELISA Kit is a powerful tool for measuring levels of interleukin-1 alpha in human samples. IL-1 alpha is a critical cytokine involved in inflammatory responses, immune regulation, and cell growth, making it essential for studying various diseases and conditions.This ELISA kit offers high sensitivity and specificity, providing accurate and reliable results for serum, plasma, and cell culture supernatants. Researchers can trust the precision of this kit for their studies in areas such as autoimmune diseases, inflammatory disorders, and cancer research.With its user-friendly protocol and quick turnaround time, the Human IL-1 Alpha ELISA Kit from Assay Genie is an excellent choice for researchers looking to delve deeper into the role of interleukin-1 alpha in human health and disease.
Product Name:
Human IL-1 alpha ELISA Kit
SKU:
HUES01374
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
2.35 pg/mL
Detection range:
3.91-250 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
