The Human IL-12 CLIA Kit is a cutting-edge diagnostic tool designed for the precise and reliable measurement of interleukin-12 levels in human samples, including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for a variety of research applications.Interleukin-12 is a key cytokine that plays a critical role in regulating immune responses, particularly in the activation of T cells and natural killer cells. Dysregulation of IL-12 has been implicated in various inflammatory and autoimmune diseases, making it a valuable biomarker for understanding the underlying mechanisms of these conditions and developing targeted therapies. Whether you are studying infectious diseases, autoimmune disorders, or immunotherapy, the Human IL-12 CLIA Kit from Assay Genie provides the precision and reliability you need to advance your research efforts. Unlock new insights into the complex interactions of the immune system with this state-of-the-art assay kit.
Product Name:
Human IL-12 CLIA Kit
SKU:
HUES00133
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
7.5 pg/mL
Detection range:
12.5-800 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
