The Human IL-33 CLIA Kit is a highly reliable and sensitive assay designed for the accurate detection of IL-33 levels in human serum, plasma, and cell culture supernatants. This kit provides researchers with the tools needed to study the role of IL-33 in various biological processes and disease states. IL-33 is a key cytokine involved in inflammation and immune responses, playing a critical role in conditions such as asthma, autoimmune diseases, and allergic reactions. By accurately measuring IL-33 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets. The Human IL-33 CLIA Kit offers high specificity and reproducibility, ensuring that researchers can confidently rely on the results obtained. Whether studying basic biology or exploring potential treatment options, this kit is an essential tool for advancing research in immunology and related fields.
Product Name:
Human IL-33 CLIA Kit
SKU:
HUES01249
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
