The Human IL6R ELISA Kit is a reliable and precise tool for detecting IL6R levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and consistent results, making it suitable for various research purposes.IL6R, also known as interleukin-6 receptor, plays a crucial role in the immune response and inflammatory processes. It is involved in various diseases, including autoimmune disorders, inflammatory conditions, and cancer. Studying IL6R levels can provide valuable insights into the mechanisms underlying these diseases and aid in the development of targeted therapies.This ELISA kit is easy to use and includes all the necessary components for performing the assay. With its exceptional performance and versatility, the Human IL6R ELISA Kit is an essential tool for researchers studying the role of IL6R in health and disease.
Product Name:
Human IL-6R ELISA Kit
SKU:
HUES01459
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
