The INS ELISA Kit is designed for the quantitative detection of Insulin (INS) levels in various human biological samples. Being a vital hormone, insulin is key for diverse cellular functions, including glucose metabolism, cellular growth, and differentiation. It plays an important role in the body's metabolic response by regulating the glucose levels in the blood, and imbalances often lead to critical health conditions like diabetes. Accurate measurement of insulin is crucial for understanding its involvement in metabolic processes and can provide key insights for strategising suitable therapeutic interventions. Assay Genie's INS ELISA Kit offers outstanding sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under rigorous quality control standards, this kit provides formidable performance and is user-friendly, making it an excellent choice for both research and clinical applications. Trust Assay Genie's INS ELISA Kit for accurate and dependable quantification of this vital biomarker in your studies.
Product Name:
Human INS (Insulin) ELISA Kit
SKU:
AEES00036
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 μIU/mL
Detection range:
0.78-50 μIU/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
