Human Interleukin 12 Receptor Beta 1 (IL-12Rb1) ELISA Kit
The Human Interleukin-12 Receptor Beta-1 (IL12RB1) ELISA Kit is a highly reliable and sensitive tool for the precise quantification of IL12RB1 levels in human samples including serum, plasma, and cell culture supernatants. With its high specificity, this kit ensures accurate and reproducible results, making it suitable for a wide array of research applications.IL12RB1 is a critical receptor involved in the immune response, specifically in the signaling pathway of interleukin-12, a cytokine that plays a key role in the regulation of immune responses.
Dysregulation of IL12RB1 has been implicated in various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and potentially identifying new therapeutic targets.Overall, the Human IL12RB1 ELISA Kit offers researchers a reliable tool for studying the role of IL12RB1 in immune regulation and disease pathogenesis, ultimately aiding in the development of novel therapeutic strategies.
Product Name:
Human Interleukin 12 Receptor Beta 1 (IL-12Rb1) ELISA Kit
Product Code:
HUDL01465
Size:
96 Assays
Target:
IL12Rb1
Synonyms:
CD212, IL12R-B1, IL12-RB1, IL-12R-Beta1, IL12RB
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-100
90
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
