Human ISG15 (Interferon Stimulated Gene 15) ELISA Kit
The Human ISG15 (Interferon-Stimulated Gene 15) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of ISG15 levels in human serum, plasma, and cell culture supernatants. This kit delivers reliable and reproducible results, making it ideal for a variety of research applications.ISG15 is a key protein in the interferon pathway, playing a crucial role in the host immune response to viral infections and other pathogens. Dysregulation of ISG15 has been implicated in various diseases, including autoimmune disorders, infectious diseases, and cancer. Therefore, the Human ISG15 ELISA Kit is an essential tool for studying these conditions and potentially developing novel therapeutic interventions.Overall, the Human ISG15 ELISA Kit offers researchers a valuable tool for investigating the role of ISG15 in health and disease, providing accurate and precise measurements for insightful research purposes.
Product Name:
Human ISG15 (Interferon Stimulated Gene 15) ELISA Kit
SKU:
HUES01468
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
