The Human KIM-1 (Kidney Injury Molecule-1) CLIA Kit is specifically designed for the accurate detection of KIM-1 levels in human biological samples such as serum and urine. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results that are essential for research and clinical applications.KIM-1 is a biomarker of kidney injury and is involved in the early detection and monitoring of renal damage. Elevated levels of KIM-1 have been associated with various kidney diseases and conditions, making it a valuable tool for diagnosing and managing renal disorders.
This CLIA kit is easy to use, providing quick and precise results that can aid in the early identification of kidney injury and guide treatment decisions. Researchers and healthcare professionals can benefit from the accurate and reliable performance of the Human KIM-1 (Kidney Injury Molecule-1) CLIA Kit in studying kidney-related diseases and developing potential therapeutic interventions.
Product Name:
Human KIM-1 (Kidney Injury Molecule 1) CLIA Kit
SKU:
HUES00160
Target:
Human KIM-1 (Kidney Injury Molecule 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human KIM-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KIM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human KIM-1. You can calculate the concentration of Human KIM-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
96-110
92-108
Average (%)
94
104
99
1:4
Range (%)
85-99
103-118
86-100
Average (%)
92
109
92
1:8
Range (%)
91-105
97-114
91-106
Average (%)
96
105
97
1:16
Range (%)
94-110
100-114
95-105
Average (%)
101
106
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
96
EDTA plasma (n=5)
94-110
100
Cell culture media (n=5)
97-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.14
183.76
955.58
98.23
189.21
1032.4
Standard deviation
7.67
15.91
66.03
12.76
20.64
85.38
C V (%)
8.15
8.66
6.91
12.99
10.91
8.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human KIM-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human KIM-1 in samples. No significant cross-reactivity or interference between Human KIM-1 and analogues was observed.
