Human LAP3 Recombinant Protein (RPPB1882)

Product Name:	Human LAP3 Recombinant Protein
Product Code:	RPPB1882
Size:	20µg
Species:	Human
Target:	LAP3
Synonyms:	Leucine Aminopeptidase 3, Peptidase S, PEPS, ProlineAminopeptidase, Leucyl Aminopeptidase, ProlylAminopeptidase, EC 3.4.11.1, LAP-3, LAPEP, Epididymis Secretory Protein Li 106,Cytosol Aminopeptidase, EC 3.4.11.5, HEL-S-106, EC 3.4.11, LAP, Cytosolaminopeptidase, Leucine aminopeptidase 3, Leucyl aminopeptidase, Prolineaminopeptidase, Prolyl aminopeptidase.
Source:	Escherichia Coli

Physical Appearance:	Sterile Filtered colorless solution.
Formulation:	LAP3 protein solution (0.5mg/ml) containing 20mMTris-HCl(pH8.5), 50% glycerol, 5mM DTT and 1mM EDTA.
Stability:	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:	Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:	MGSSHHHHHH SSGLVPRGSHMFLLPLPAAG RVVVRRLAVR RFGSRSLSTA DMTKGLVLGI YSKEKEDDVP QFTSAGENFD KLLAGKLRETLNISGPPLKA GKTRTFYGLH QDFPSVVLVG LGKKAAGIDE QENWHEGKEN IRAAVAAGCR QIQDLELSSVEVDPCGDAQA AAEGAVLGLY EYDDLKQKKK MAVSAKLYGS GDQEAWQKGV LFASGQNLAR QLMETPANEMTPTRFAEIIE KNLKSASSKT EVHIRPKSWI EEQAMGSFLS VAKGSDEPPV FLEIHYKGSP NANEPPLVFVGKGITFDSGG ISIKASANMD LMRADMGGAA TICSAIVSAA KLNLPINIIG LAPLCENMPS GKANKPGDVVRAKNGKTIQV DNTDAEGRLI LADALCYAHT FNPKVILNAA TLTGAMDVAL GSGATGVFTN SSWLWNKLFEASIETGDRVW RMPLFEHYTR QVVDCQLADV NNIGKYRSAG ACTAAAFLKE FVTHPKWAHL DIAGVMTNKDEVPYLRKGMT GRPTRTLIEF LLRFSQDNA

Leucine Aminopeptidase 3 also known as LAP3 is a member of the peptidaseM17 family. LAP3 is implicated in the processing as well as regular turnover ofintracellular proteins. LAP3 catalyzes the removal of unsubstituted N-terminalamino acids from different peptides. LAP3is responsible of releasing an N-terminal amino acid, Xaa-|-Yaa-, in which Xaais preferably Leu, but could be other amino acids including Pro although notArg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are as wellreadily hydrolyzed; however rates on arylamides are exceedingly low.

LAP3 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 539 amino acids (1-519 a.a) andhaving a molecular mass of 58.3kDa.LAP3 is fused to a 20 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.

UniProt Protein Function:	LAP3: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. Belongs to the peptidase M17 family. 2 isoforms of the human protein are produced by alternative initiation.
UniProt Protein Details:	Protein type:Other Amino Acids Metabolism - glutathione; Protease; EC 3.4.11.5; Mitochondrial; EC 3.4.11.1; Amino Acid Metabolism - arginine and proline Chromosomal Location of Human Ortholog: 4p15.32 Cellular Component: cytoplasm; focal adhesion; nucleoplasm; nucleus
UniProt Code:	P28838
NCBI GenInfo Identifier:	124028615
NCBI Gene ID:	51056
NCBI Accession:	P28838.3
UniProt Secondary Accession:	P28838,Q6IAM6, Q6P0L6, Q9UQE3, B3KMQ3,
UniProt Related Accession:	P28838
Molecular Weight:	52,771 Da
NCBI Full Name:	Cytosol aminopeptidase
NCBI Synonym Full Names:	leucine aminopeptidase 3
NCBI Official Symbol:	LAP3
NCBI Official Synonym Symbols:	LAP; PEPS; LAPEP; HEL-S-106
NCBI Protein Information:	cytosol aminopeptidase
UniProt Protein Name:	Cytosol aminopeptidase
UniProt Synonym Protein Names:	Leucine aminopeptidase 3; LAP-3; Leucyl aminopeptidase; Peptidase S; Proline aminopeptidase (EC:3.4.11.5); Prolyl aminopeptidase
Protein Family:	Leucine aminopeptidase
UniProt Gene Name:	LAP3
UniProt Entry Name:	AMPL_HUMAN

Izquierdo et al.

Identification of a potent and selective LAPTc inhibitor by RapidFire-Mass Spectrometry, with antichagasic activity

PLOS Neglected Tropical Diseases 2024

PubMed ID: 38359089