The LEP (Leptin) ELISA Kit is specially formulated for the precise and quantitative detection of Leptin levels in various human biological samples. Leptin, a crucial hormone produced by adipose tissue, regulates energy balance, metabolism, and appetite. Its key role in metabolic processes and signaling makes it an essential biomarker for understanding metabolic disorders and obesity-related conditions. With exceptional sensitivity and specificity, the LEP ELISA Kit offers reliable and reproducible results. Manufactured to meet stringent quality control standards, this kit ensures robust performance and user-friendly operation, catering to both research and clinical applications. Put your trust in Assay Genie's LEP ELISA Kit for accurate and dependable quantification of this fundamental biomarker in your research studies.
Product Name:
Human LEP (Leptin) ELISA Kit
SKU:
AEES00053
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
