Description
| Product Name: | Human MCTS1 Recombinant Protein |
| Product Code: | RPPB3947 |
| Size: | 20µg |
| Species: | Human |
| Target: | MCTS1 |
| Synonyms: | MCT-1, MCT1, Malignant T-cell-amplified sequence 1, Multiple copies T-cell malignancies. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | MCTF1 protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMFKKFDE KENVSNCIQL KTSVIKGIKN QLIEQFPGIE PWLNQIMPKK DPVKIVRCHE HIEILTVNGE LLFFRQREGP FYPTLRLLHK YPFILPHQQV DKGAIKFVLS GANIMCPGLT SPGAKLYPAA VDTIVAIMAE GKQHALCVGV MKMSAEDIEK VNKGIGIENI HYLNDGLWHM KTYK |
Malignant T cell amplified sequence 1 (MCTS1) is an Anti-oncogene which takes part in cell cycle regulation by means of decreasing cell doubling time and encouraging-dependent growth and also by shorting the duration of G1 transit time and G1/S transition. MCTS1 also plays a role as translation enhancer. MCTS1 recruits the density-regulated protein/DENR and binds to the cap complex of the 5'-terminus of mRNAs, thereupon altering the mRNA translation profile; Up-regulates protein levels of BCL2L2, TFDP1, MRE11A, CCND1 and E2F1, while mRNA levels remains constant.
MCTF1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 204 amino acids (1-181 a.a.) and having a molecular mass of 22.9kDa. MCTF1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MCTS1: Anti-oncogene that play a role in cell cycle regulation; decreases cell doubling time and anchorage-dependent growth; shortens the duration of G1 transit time and G1/S transition. When constituvely expressed, increases CDK4 and CDK6 kinases activity and CCND1/cyclin D1 protein level, as well as G1 cyclin/CDK complex formation. Plays a role as translation enhancer; Recruits the density-regulated protein/DENR and binds to the cap complex of the 5'-terminus of mRNAs, subsequently altering the mRNA translation profile; Up-regulates protein levels of BCL2L2, TFDP1, MRE11A, CCND1 and E2F1, while mRNA levels remains constant. Hyperactivates DNA damage signaling pathway; increased gamma- irradiation-induced phosphorylation of histone H2AX, and induces damage foci formation. Increases the overall number of chromosomal abnormalities such as larger chromosomes formation and multiples chromosomal fusions when overexpressed in gamma-irradiated cells. May play a role in promoting lymphoid tumor development: lymphoid cell lines overexpressing MCTS1 exhibit increased growth rates and display increased protection against apoptosis. May contribute to the pathogenesis and progression of breast cancer via promotion of angiogenesis through the decline of inhibitory THBS1/thrombospondin-1, and inhibition of apoptosis. Involved in the process of proteasome degradation to down-regulate Tumor suppressor p53/TP53 in breast cancer cell; Positively regulates phosphorylation of MAPK1 and MAPK3. Belongs to the MCTS1 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Tumor suppressor Chromosomal Location of Human Ortholog: Xq24 Cellular Component: cytoplasm; plasma membrane Biological Process: formation of translation preinitiation complex; positive regulation of cell proliferation; ribosome disassembly |
| UniProt Code: | Q9ULC4 |
| NCBI GenInfo Identifier: | 74735052 |
| NCBI Gene ID: | 28985 |
| NCBI Accession: | Q9ULC4.1 |
| UniProt Secondary Accession: | Q9ULC4,Q502X6, B4DGY2, |
| UniProt Related Accession: | Q9ULC4 |
| Molecular Weight: | 20,550 Da |
| NCBI Full Name: | Malignant T-cell-amplified sequence 1 |
| NCBI Synonym Full Names: | MCTS1, re-initiation and release factor |
| NCBI Official Symbol: | MCTS1�� |
| NCBI Official Synonym Symbols: | MCT1; MCT-1�� |
| NCBI Protein Information: | malignant T-cell-amplified sequence 1 |
| UniProt Protein Name: | Malignant T-cell-amplified sequence 1 |
| UniProt Synonym Protein Names: | Multiple copies T-cell malignancies |
| Protein Family: | Malignant T-cell-amplified sequence |
| UniProt Gene Name: | MCTS1�� |
| UniProt Entry Name: | MCTS1_HUMAN |
