Human MIF (Macrophage Migration Inhibitory Factor) ELISA Kit
The Human MIF (Macrophage Migration Inhibitory Factor) ELISA Kit is expertly designed to enable the precise quantitative analysis of Macrophage Migration Inhibitory Factor (MIF) levels in a variety of biological samples. MIF is a pivotal pro-inflammatory cytokine known for its multifaceted roles in modulating immune responses, cell migration, and inflammation processes.
It serves as a critical regulator of the body's physiological responses to various stressors, infections, and diseases, thereby influencing inflammatory cascades and immune cell activities. Our MIF ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible measurement of MIF concentrations. With stringent quality control protocols in place, this kit delivers robust and reliable performance, making it an ideal choice for research applications. Trust Assay Genie's MIF ELISA Kit for dependable quantification of this essential cytokine in your research endeavors.
Product Name:
Human MIF (Macrophage Migration Inhibitory Factor) ELISA Kit
SKU:
AEES00171
Target:
Human MIF (Macrophage Migration Inhibitory Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MIF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MIF. You can calculate the concentration of Human MIF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-111
90-100
94-105
Average (%)
105
95
100
1:4
Range (%)
96-110
86-98
91-105
Average (%)
101
92
97
1:8
Range (%)
96-112
92-103
95-107
Average (%)
102
98
101
1:16
Range (%)
96-114
91-106
96-111
Average (%)
104
97
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
92
EDTA plasma (n=5)
92-108
99
Cell culture media (n=5)
92-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.39
230.7
765.25
98.41
251.5
702.31
Standard deviation
6.11
12.94
25.64
5.69
13.73
30.76
C V (%)
6.09
5.61
3.35
5.78
5.46
4.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MIF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MIF in samples. No significant cross-reactivity or interference between Human MIF and analogues was observed.
