Human MMP-10 (Matrix Metalloproteinase 10) ELISA Kit
The Matrix Metalloproteinase 10 (MMP-10) ELISA Kit is meticulously developed for the precise quantitative analysis of MMP-10 levels in various human samples. MMP-10, an important member of the MMP family of enzymes, plays a key role in extracellular matrix remodeling and tissue homeostasis. As a biomarker of tissue remodeling and inflammation, MMP-10 is implicated in various pathological processes, including cancer progression, cardiovascular diseases, and tissue repair mechanisms.
This ELISA kit ensures exceptional sensitivity and specificity, enabling the accurate and reproducible measurement of MMP-10 levels. Manufactured under strict quality control procedures, the kit delivers robust and reliable performance while being user-friendly. Count on Assay Genie's MMP-10 ELISA Kit for precise and consistent quantification of this critical biomarker in your research and clinical investigations.
Product Name:
Human MMP-10 (Matrix Metalloproteinase 10) ELISA Kit
SKU:
AEES00058
Target:
Human MMP-10 (Matrix Metalloproteinase 10)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-10. You can calculate the concentration of Human MMP-10 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
93-106
85-99
Average (%)
98
101
91
1:4
Range (%)
85-93
91-104
91-100
Average (%)
90
96
97
1:8
Range (%)
88-98
88-101
83-94
Average (%)
94
93
91
1:16
Range (%)
84-97
87-97
92-101
Average (%)
92
93
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-111
103
EDTA plasma (n=5)
88-100
92
Cell culture media (n=5)
85-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
86.63
201.29
992.03
92.01
206.35
879.1
Standard deviation
4.63
9.64
49.4
4.77
9.51
39.12
C V (%)
5.34
4.79
4.98
5.18
4.61
4.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MMP-10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-10 in samples. No significant cross-reactivity or interference between Human MMP-10 and analogues was observed.
