The Human MMP-3 (Matrix Metalloproteinase-3) CLIA Kit is a reliable tool for the quantitative detection of MMP-3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Matrix Metalloproteinase-3, also known as stromelysin-1, is an important enzyme involved in tissue remodeling and degradation of extracellular matrix proteins.
Elevated levels of MMP-3 have been associated with various pathological conditions, including cancer, arthritis, and cardiovascular diseases.By accurately measuring MMP-3 levels, researchers can better understand the role of this enzyme in disease progression and potentially identify new therapeutic targets. The Human MMP-3 CLIA Kit is a valuable tool for studying the biology of MMP-3 and developing novel treatment strategies for related disorders.
Product Name:
Human MMP-3 (Matrix Metalloproteinase 3) CLIA Kit
SKU:
HUES00823
Target:
Human MMP-3 (Matrix Metalloproteinase 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MMP-3. You can calculate the concentration of Human MMP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
98-112
92-104
Average (%)
103
105
98
1:4
Range (%)
95-110
88-99
96-112
Average (%)
101
94
102
1:8
Range (%)
103-120
94-106
103-116
Average (%)
109
99
110
1:16
Range (%)
95-108
96-112
88-102
Average (%)
101
103
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-113
107
EDTA plasma (n=5)
87-98
93
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.7
311.19
682.01
111.19
301.41
673.14
Standard deviation
9.02
30.53
48.97
10.16
22.36
74.92
C V (%)
8.45
9.81
7.18
9.14
7.42
11.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MMP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-3 in samples. No significant cross-reactivity or interference between Human MMP-3 and analogues was observed.
