The Human MYCBP (c-Myc Binding Protein) CLIA Kit is specifically designed for the precise quantification of MYCBP levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for various research purposes.MYCBP is a key protein that binds to the c-Myc oncogene, regulating its activity and influencing cell growth and proliferation. Dysregulation of the c-Myc-MYCBP interaction has been implicated in various diseases, including cancer and genetic disorders, highlighting the importance of studying MYCBP levels as a potential biomarker.
With its advanced technology and easy-to-use format, the Human MYCBP CLIA Kit is a valuable tool for researchers investigating the role of MYCBP in disease pathology and exploring potential therapeutic interventions. Invest in this kit today to accelerate your research and advance scientific discovery.
Product Name:
Human MYCBP (C-Myc Binding Protein) CLIA Kit
SKU:
HUES00517
Target:
Human MYCBP (C-Myc Binding Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MYCBP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MYCBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MYCBP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MYCBP. You can calculate the concentration of Human MYCBP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
101-114
93-107
Average (%)
97
107
98
1:4
Range (%)
91-106
101-116
100-114
Average (%)
97
108
107
1:8
Range (%)
99-112
89-102
98-114
Average (%)
105
96
107
1:16
Range (%)
97-110
89-100
101-118
Average (%)
104
95
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
95-109
101
Cell culture media (n=5)
90-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.39
218.14
946.18
109.08
200.75
992.51
Standard deviation
13.34
20.18
95.0
12.47
16.44
87.54
C V (%)
12.08
9.25
10.04
11.43
8.19
8.82
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MYCBP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MYCBP in samples. No significant cross-reactivity or interference between Human MYCBP and analogues was observed.
