Human NAA10 Recombinant Protein (RPPB1990)
- SKU:
- RPPB1990
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P41227
- Research Area:
- Enzymes
Description
| Product Name: | Human NAA10 Recombinant Protein |
| Product Code: | RPPB1990 |
| Size: | 20µg |
| Species: | Human |
| Target: | NAA10 |
| Synonyms: | N-alpha-acetyltransferase 10, N-terminal acetyltransferase complex ARD1 subunit homolog A, NatA catalytic subunit, NAA10, ARD1, ARD1A, TE2, NATD, DXS707. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | NAA10 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 5mM DTT, 10% glycerol and 200mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MNIRNARPED LMNMQHCNLL CLPENYQMKY YFYHGLSWPQ LSYIAEDENG KIVGYVLAKM EEDPDDVPHG HITSLAVKRS HRRLGLAQKL MDQASRAMIE NFNAKYVSLH VRKSNRAALH LYSNTLNFQI SEVEPKYYAD GEDAYAMKRD LTQMADELRR HLELKEKGRH VVLGAIENKV ESKGNSPPSS GEACREEKGL AAEDSGGDSK DLSEVSETTE STDVKDSSEA SDSAS |
NAA10 is a member of the acetyltransferase family. NAA10 interacts with NAA15, HIF-1 and with the ribosome. In its binding to HIF-1, NAA10 functions as a protein acetyltransferase by regulating its stability. In various cell lines, NAA10 is downregulated in response to hypoxia. NAA10 is expressed during the course of the development of the brain.
NAA10 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 255 amino acids (1-235 a.a.) and having a molecular mass of 28.6kDa (the molecular weight on SDS-PAGE will appear higher).NAA10 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NAA10: In complex with NAA15, displays alpha (N-terminal) acetyltransferase activity. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration. Interacts with HIF1A (via its ODD domain); the interaction increases HIF1A protein stability during normoxia, and down-regulates it when induced by hypoxia. Interacts with NAA15, NAA50 and with the ribosome. Binds to MYLK. Ubiquitous. Belongs to the acetyltransferase family. ARD1 subfamily. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - tyrosine; Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - phenylalanine; EC 2.3.1.88; Lipid Metabolism - glycerophospholipid; Acetyltransferase Chromosomal Location of Human Ortholog: Xq28 Cellular Component: membrane; cytoplasm; nucleolus; intracellular; nucleus Molecular Function:peptide alpha-N-acetyltransferase activity; N-acetyltransferase activity; protein binding; acetyltransferase activity; ribosome binding Biological Process: N-terminal protein amino acid acetylation; N-terminal peptidyl-glutamic acid acetylation; internal protein amino acid acetylation; N-terminal peptidyl-serine acetylation; DNA packaging Disease: Ogden Syndrome; Microphthalmia, Syndromic 1 |
| NCBI Summary: | N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012] |
| UniProt Code: | P41227 |
| NCBI GenInfo Identifier: | 728880 |
| NCBI Gene ID: | 8260 |
| NCBI Accession: | P41227.1 |
| UniProt Secondary Accession: | P41227,A6NM98, |
| UniProt Related Accession: | P41227 |
| Molecular Weight: | 235 |
| NCBI Full Name: | N-alpha-acetyltransferase 10 |
| NCBI Synonym Full Names: | N(alpha)-acetyltransferase 10, NatA catalytic subunit |
| NCBI Official Symbol: | NAA10�� |
| NCBI Official Synonym Symbols: | TE2; ARD1; NATD; ARD1A; ARD1P; DXS707; MCOPS1�� |
| NCBI Protein Information: | N-alpha-acetyltransferase 10; natA catalytic subunit Naa10; ARD1 homolog A, N-acetyltransferase; N-acetyltransferase ARD1, human homolog of; N-alpha-acetyltransferase 10, NatA catalytic subunit; N-terminal acetyltransferase complex ARD1 subunit homolog A |
| UniProt Protein Name: | N-alpha-acetyltransferase 10 |
| UniProt Synonym Protein Names: | N-terminal acetyltransferase complex ARD1 subunit homolog A; NatA catalytic subunit Naa10 |
| Protein Family: | N-alpha-acetyltransferase |
| UniProt Gene Name: | NAA10�� |
| UniProt Entry Name: | NAA10_HUMAN |
