Human NFE2L2 (Nuclear Factor, Erythroid Derived 2 Like 2) CLIA Kit
Based on the information provided in the URL, we have a product description for the Human NFE2L2 (Nuclear factor erythroid 2 like 2) CLIA Kit:The Human NFE2L2 (Nuclear factor erythroid 2 like 2) CLIA Kit is specifically designed for the precise measurement of NFE2L2 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.NFE2L2 is a critical transcription factor involved in cellular defense mechanisms and the regulation of antioxidant response elements.
It plays a crucial role in maintaining cellular homeostasis and protecting cells from oxidative stress, inflammation, and other pathological conditions. As a key player in oxidative stress-related diseases such as cancer, neurodegenerative disorders, and metabolic syndromes, NFE2L2 serves as a valuable biomarker for studying disease mechanisms and exploring therapeutic interventions.With its high performance and versatility, the Human NFE2L2 CLIA Kit is an essential tool for researchers investigating the role of NFE2L2 in disease pathology and developing novel strategies for therapeutic interventions.
Product Name:
Human NFE2L2 (Nuclear Factor, Erythroid Derived 2 Like 2) CLIA Kit
SKU:
HUES00889
Target:
Human NFE2L2 (Nuclear Factor, Erythroid Derived 2 Like 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NFE2L2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NFE2L2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NFE2L2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NFE2L2. You can calculate the concentration of Human NFE2L2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-117
94-106
93-106
Average (%)
108
100
100
1:4
Range (%)
103-115
102-119
100-118
Average (%)
109
108
108
1:8
Range (%)
93-105
95-110
100-114
Average (%)
99
101
106
1:16
Range (%)
85-98
93-110
95-106
Average (%)
90
100
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
105
EDTA plasma (n=5)
100-114
107
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.03
295.14
683.64
100.62
322.59
743.07
Standard deviation
11.37
33.29
63.99
10.9
28.36
66.65
C V (%)
10.43
11.28
9.36
10.83
8.79
8.97
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NFE2L2 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human NFE2L2 in samples. No significant cross-reactivity or interference between Human NFE2L2 and analogues was observed.
