Human NNT1 Recombinant Protein (RPPB0784)
- SKU:
- RPPB0784
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9UBD9
- Research Area:
- Neurotrophins
Description
| Product Name: | Human NNT1 Recombinant Protein |
| Product Code: | RPPB0784 |
| Size: | 10µg |
| Species: | Human |
| Target: | NNT1 |
| Synonyms: | Cardiotrophin-Like Cytokine Factor 1, Novel Neurotrophin-1 , BSF3, CLC, CRLF1 Associated Cytokine-Like Factor 1, B-Cell Stimulating Factor 3, B-Cell-Stimulating Factor 3, BSF-3, NNT-1, NNT1, Neurotrophin-1/B-Cell Stimulating Factor-3, Cold-Induced Sweating Syndrome 2, B-Cell Stimulatory Factor 3, CISS2, NR6, CLCF1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | NNT-1 protein was lyophilized from a 0.2�m filtered solution in Acetonitrile and TFA. |
| Solubility: | It is recommended to reconstitute the lyophilized NNT1 in sterile 18M-cm H2O not less than 0.5mg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Neurotrophin-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NNT1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF |
| Biological Activity: | The ED50, as measured in a cell proliferation assay using human TF?1 cells transfected with human CNTF R?, is less than 15ng/ml. |
Cardiotrophin-like cytokine (CLC/ NNT-1) belongs to the IL-6 family of cytokines. All family members share the receptor subunit gp130, which belongs to the type I cytokine receptor superfamily. NNT1 is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs, and an inducer of IgE synthesis and B cell proliferation. Cells expressing NNT-1 include embryonic muscle, lung epithelium, and mesenchyme. NNT1 binds to and activates the ILST/gp130 receptor.
Neurotrophin-1 Human Recombinant (28-225) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 199 amino acids and having a molecular mass of 22kDa.The NNT-1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CLCF1: Cytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor. Defects in CLCF1 are the cause of cold-induced sweating syndrome type 2 (CISS2). Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high- arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Apoptosis; Cytokine; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 11q13.3 Cellular Component: extracellular region Molecular Function:ciliary neurotrophic factor receptor binding; cytokine activity; growth factor activity; protein binding; protein heterodimerization activity; receptor binding Biological Process: B cell differentiation; cell surface receptor linked signal transduction; JAK-STAT cascade; negative regulation of neuron apoptosis; positive regulation of astrocyte differentiation; positive regulation of B cell proliferation; positive regulation of cell proliferation; positive regulation of immunoglobulin production; positive regulation of isotype switching to IgE isotypes; positive regulation of tyrosine phosphorylation of Stat3 protein Disease: Cold-induced Sweating Syndrome 2 |
| NCBI Summary: | This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009] |
| UniProt Code: | Q9UBD9 |
| NCBI GenInfo Identifier: | 56404673 |
| NCBI Gene ID: | 23529 |
| NCBI Accession: | Q9UBD9.1 |
| UniProt Secondary Accession: | Q9UBD9,Q6NZA4, B4DNT4, |
| UniProt Related Accession: | Q9UBD9 |
| Molecular Weight: | 24,087 Da |
| NCBI Full Name: | Cardiotrophin-like cytokine factor 1 |
| NCBI Synonym Full Names: | cardiotrophin-like cytokine factor 1 |
| NCBI Official Symbol: | CLCF1�� |
| NCBI Official Synonym Symbols: | CLC; NR6; BSF3; NNT1; BSF-3; CISS2; NNT-1�� |
| NCBI Protein Information: | cardiotrophin-like cytokine factor 1 |
| UniProt Protein Name: | Cardiotrophin-like cytokine factor 1 |
| UniProt Synonym Protein Names: | B-cell-stimulating factor 3; BSF-3; Novel neurotrophin-1; NNT-1 |
| Protein Family: | Cardiotrophin-like cytokine factor |
| UniProt Gene Name: | CLCF1�� |
| UniProt Entry Name: | CLCF1_HUMAN |
