Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) ELISA Kit
The Human NOS3 (eNOS) ELISA Kit is specifically designed for the precise quantification of nitric oxide synthase 3 (NOS3) levels in human endothelial cells, serum, and plasma samples. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research experiments.NOS3, also known as endothelial nitric oxide synthase (eNOS), is a critical enzyme involved in the production of nitric oxide, a key signaling molecule in the cardiovascular system.
Dysregulation of NOS3 activity has been linked to various cardiovascular diseases, such as hypertension and atherosclerosis, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.By using the Human NOS3 ELISA Kit, researchers can gain valuable insights into the role of NOS3 in endothelial function and cardiovascular health, paving the way for innovative research and treatment strategies in the field of cardiovascular medicine.
Product Name:
Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) ELISA Kit
SKU:
HUES01899
Target:
Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NOS3/eNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NOS3/eNOS. You can calculate the concentration of Human NOS3/eNOS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
98-112
95-111
Average (%)
99
104
101
1:4
Range (%)
98-115
88-101
97-114
Average (%)
106
93
104
1:8
Range (%)
97-113
81-93
98-113
Average (%)
104
87
104
1:16
Range (%)
98-111
83-95
90-106
Average (%)
104
88
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
101
EDTA plasma (n=5)
94-106
99
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
186.7
451.7
1380.6
204.8
422.5
1440.9
Standard deviation
12.9
20.3
69.0
12.7
18.6
46.1
C V (%)
6.91
4.49
5.0
6.2
4.4
3.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NOS3/eNOS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NOS3/eNOS in samples. No significant cross-reactivity or interference between Human NOS3/eNOS and analogues was observed.
