The Human NPY (Neuropeptide Y) ELISA Kit is specifically designed for the precise measurement of neuropeptide Y levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit offers reliable and reproducible results, making it a valuable tool for various research applications.Neuropeptide Y is a neurotransmitter that plays a crucial role in regulating appetite, stress response, and cardiovascular functions.
Dysregulation of neuropeptide Y has been linked to various diseases such as obesity, anxiety disorders, and hypertension, making it a key biomarker for studying these conditions and developing potential therapies.Overall, the Human NPY (Neuropeptide Y) ELISA Kit provides researchers with a robust and accurate means of quantifying neuropeptide Y levels, aiding in the advancement of research in the field of neuroscience and related disciplines.
Product Name:
Human NPY (Neuropeptide Y) ELISA Kit
SKU:
HUES02841
Target:
Human NPY (Neuropeptide Y)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with NPY. During the reaction, NPY in the sample or standard competes with a fixed amount of NPY on the solid phase supporter for sites on the Biotinylated Detection Ab specific to NPY. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NPY in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
97-111
90-102
Average (%)
102
105
95
1:4
Range (%)
88-101
91-106
100-114
Average (%)
95
99
106
1:8
Range (%)
84-96
90-102
97-110
Average (%)
90
95
102
1:16
Range (%)
89-102
86-101
92-107
Average (%)
96
93
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
91
EDTA plasma (n=5)
92-106
100
Cell culture media (n=5)
87-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.8
187.0
734.3
100.0
174.5
759.2
Standard deviation
6.6
8.2
25.0
6.2
7.7
29.6
C V (%)
6.61
4.39
3.4
6.2
4.41
3.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of NPY concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes NPY in samples. No significant cross-reactivity or interference between NPY and analogues was observed.
