The Human NRP1 (Neuropilin 1) ELISA Kit is expertly designed for the accurate quantification of Neuropilin 1 (NRP1) levels in various biological samples.
NRP1 is an essential transmembrane receptor that plays versatile roles in various cellular functions, including angiogenesis, neuronal development, immune regulation, and cancer progression. As a multifunctional receptor involved in signaling pathways, NRP1 contributes significantly to physiological processes and pathologies, making it a key target for research in multiple fields. Our NRP1 ELISA Kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results that meet the highest standards of accuracy. Manufactured under stringent quality controls, this kit guarantees robust performance and ease of use, making it the ideal choice for leading-edge research applications. Trust Assay Genie's NRP1 ELISA Kit for reliable and dependable quantification of this crucial receptor in your scientific inquiries.
Product Name:
Human NRP1 (Neuropilin 1) ELISA Kit
SKU:
AEES00167
Target:
Human NRP1 (Neuropilin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NRP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NRP1. You can calculate the concentration of Human NRP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-102
95-111
93-107
Average (%)
93
101
100
1:4
Range (%)
84-95
91-102
92-105
Average (%)
88
97
97
1:8
Range (%)
85-100
89-103
90-103
Average (%)
92
97
95
1:16
Range (%)
89-100
95-110
93-108
Average (%)
95
102
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-107
98
EDTA plasma (n=5)
84-95
90
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.49
119.92
425.81
59.43
125.36
427.44
Standard deviation
3.81
5.35
21.76
4.08
5.73
22.4
C V (%)
7
4.46
5.11
6.86
4.57
5.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NRP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NRP1 in samples. No significant cross-reactivity or interference between Human NRP1 and analogues was observed.
