The Human NRP2 (Neuropilin-2) ELISA Kit is a research tool designed for the precise measurement of neuropilin-2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications.Neuropilin-2 is a key protein involved in various biological processes, including angiogenesis, axon guidance, and immune response modulation. Its dysregulation has been implicated in a range of diseases, including cancer, autoimmune disorders, and neurological conditions, making it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions. For researchers investigating the role of neuropilin-2 in health and disease, the Human NRP2 ELISA Kit is an indispensable tool for reliable and reproducible measurements, enabling a deeper understanding of the complex pathways involving this important protein.
Product Name:
Human NRP2 (Neuropilin 2) ELISA Kit
SKU:
HUES02880
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.