The Human NT-3 (Neurotrophin-3) CLIA Kit is specially designed for the accurate measurement of neurotrophin-3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Neurotrophin-3 is a critical growth factor that plays a key role in the development and maintenance of the nervous system. It is involved in promoting the survival and differentiation of neurons, making it a crucial biomarker for studying neurological conditions and potential treatment options. By using the Human NT-3 CLIA Kit, researchers can gain valuable insights into the role of neurotrophin-3 in neurological disorders such as Alzheimer's disease, Parkinson's disease, and spinal cord injuries. With its reliable performance and accuracy, this kit is an essential tool for advancing our understanding of neurotrophin-3 biology and developing innovative therapeutic approaches.
Product Name:
Human NT-3 (Neurotrophin-3) CLIA Kit
SKU:
HUES01045
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
