Human NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) CLIA Kit
The Human NT-proBNP (N-Terminal pro-Brain Natriuretic Peptide) CLIA Kit is specifically designed for the accurate measurement of NT-proBNP levels in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results for a variety of research applications.NT-proBNP is a biomarker that plays a crucial role in heart function and cardiovascular diseases. This peptide is released from the heart in response to increased cardiac stress and is used as a diagnostic tool for conditions such as heart failure, myocardial infarction, and hypertension.
By accurately measuring NT-proBNP levels, researchers can gain valuable insights into cardiac health and disease progression, leading to advancements in diagnostic and therapeutic strategies. Order the Human NT-proBNP CLIA Kit today to enhance your research efforts in cardiovascular medicine.
Product Name:
Human NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) CLIA Kit
SKU:
HUES00541
Target:
Human NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NT-proBNP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NT-proBNP. You can calculate the concentration of Human NT-proBNP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
87-98
92-107
Average (%)
100
92
99
1:4
Range (%)
96-110
96-114
96-113
Average (%)
102
104
104
1:8
Range (%)
96-113
94-112
92-108
Average (%)
103
102
99
1:16
Range (%)
87-100
103-119
96-110
Average (%)
92
109
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
91
EDTA plasma (n=5)
102-116
108
Cell culture media (n=5)
94-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.1
155.35
408.42
46.44
148.56
430.49
Standard deviation
4.7
13.34
43.7
4.61
14.26
33.49
C V (%)
9.98
8.59
10.7
9.93
9.6
7.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NT-proBNP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NT-proBNP in samples. No significant cross-reactivity or interference between Human NT-proBNP and analogues was observed.
