Human NT5E (CD73) Recombinant Protein (RPPB4128)
- SKU:
- RPPB4128
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P21589
- Research Area:
- CD Antigens
Description
| Product Name: | Human NT5E (CD73) Recombinant Protein |
| Product Code: | RPPB4128 |
| Size: | 10µg |
| Species: | Human |
| Target: | NT5E (CD73) |
| Synonyms: | NT, eN, NT5, NTE, eNT, CD73, E5NT, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, NT5E. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | NT5E (CD73) protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MWELTILHTN DVHSRLEQTS EDSSKCVNAS RCMGGVARLF TKVQQIRRAE PNVLLLDAGD QYQGTIWFTV YKGAEVAHFM NALRYDAMAL GNHEFDNGVE GLIEPLLKEA KFPILSANIK AKGPLASQIS GLYLPYKVLP VGDEVVGIVG YTSKETPFLS NPGTNLVFED EITALQPEVD KLKTLNVNKI IALGHSGFEM DKLIAQKVRG VDVVVGGHSN TFLYTGN |
NT5E, also known as CD73, is a plasma membrane protein which catalyzes the conversion of extracellular nucleotides into membrane-permeable nucleosides. NT5E is functions as a determinant of lymphocyte differentiation. Defects in NT5E can cause the calcification of joints and arteries.��
NT5E (CD73) Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 247amino acids (27-252 a.a) and having a molecular mass of 27kDa. NT5E (CD73) is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.1.3.5; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Phosphatase (non-protein); Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide Chromosomal Location of Human Ortholog: 6q14-q21 Cellular Component: cell surface; membrane; cytoplasm; plasma membrane Molecular Function:5'-nucleotidase activity; metal ion binding; nucleotide binding Biological Process: pyrimidine nucleoside catabolic process; dephosphorylation; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process Disease: Calcification Of Joints And Arteries |
| NCBI Summary: | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] |
| UniProt Code: | P21589 |
| NCBI GenInfo Identifier: | 112825 |
| NCBI Gene ID: | 4907 |
| NCBI Accession: | P21589.1 |
| UniProt Related Accession: | P21589 |
| Molecular Weight: | |
| NCBI Full Name: | 5'-nucleotidase |
| NCBI Synonym Full Names: | 5'-nucleotidase ecto |
| NCBI Official Symbol: | NT5E�� |
| NCBI Official Synonym Symbols: | NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA�� |
| NCBI Protein Information: | 5'-nucleotidase |
| UniProt Protein Name: | 5'-nucleotidase |
| UniProt Synonym Protein Names: | Ecto-5'-nucleotidase; CD_antigen: CD73 |
| UniProt Gene Name: | NT5E�� |
| UniProt Entry Name: | 5NTD_HUMAN |
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