Human NTHL1 Recombinant Protein (RPPB2042)
- SKU:
- RPPB2042
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P78549
- Research Area:
- Enzymes
Description
| Product Name: | Human NTHL1 Recombinant Protein |
| Product Code: | RPPB2042 |
| Size: | 10µg |
| Species: | Human |
| Target: | NTHL1 |
| Synonyms: | Endonuclease III-like protein 1, NTHL1, NTH1, OCTS3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | NTHL1 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 80.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MCSPQESGMT ALSARMLTRS RSLGPGAGPR GCREEPGPLR RREAAAEARK SHSPVKRPRK AQRLRVAYEG SDSEKGEGAE PLKVPVWEPQ DWQQQLVNIR AMRNKKDAPV DHLGTEHCYD SSAPPKVRRY QVLLSLMLSS QTKDQVTAGA MQRLRARGLT VDSILQTDDA TLGKLIYPVG FWRSKVKYIK QTSAILQQHY GGDIPASVAE LVALPGVGPK MAHLAMAVAW GTVSGIAVDT HVHRIANRLR WTKKATKSPE ETRAALEEWL PRELWHEING LLVGFGQQTC LPVHPRCHAC LNQALCPAAQ GL |
Nth endonuclease III-like protein 1 (NTHL1) is one of the enzymes which act on a large number of oxidatively damaged DNA sites. NTHL1 has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. NTHL1 acts on a damaged strand, 5' from the damaged site and incises damaged DNA at cytosines, thymines and guanines.
NTHL1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 332 amino acids (1-312 a.a.) and having a molecular mass of 36.6kDa.NTHL1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NTHL1: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. Belongs to the Nth/MutY family. |
| UniProt Protein Details: | Protein type:DNA repair, damage; Mitochondrial; Lyase; EC 4.2.99.18; Hydrolase Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: mitochondrion; nucleoplasm; nucleus Molecular Function:4 iron, 4 sulfur cluster binding; DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) lyase activity; double-stranded DNA binding; endonuclease activity; metal ion binding; oxidized purine base lesion DNA N-glycosylase activity; oxidized pyrimidine base lesion DNA N-glycosylase activity; protein binding Biological Process: base-excision repair, AP site formation; depyrimidination; nucleotide-excision repair, DNA incision, 5'-to lesion; positive regulation of defense response to virus by host Disease: Familial Adenomatous Polyposis 3 |
| NCBI Summary: | The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity. [provided by RefSeq, Oct 2008] |
| UniProt Code: | P78549 |
| NCBI GenInfo Identifier: | 29840795 |
| NCBI Gene ID: | 4913 |
| NCBI Accession: | P78549.2 |
| UniProt Secondary Accession: | P78549,Q1MVR1, Q99566, Q99794, Q9BPX2, |
| UniProt Related Accession: | P78549 |
| Molecular Weight: | 32,839 Da |
| NCBI Full Name: | Endonuclease III-like protein 1 |
| NCBI Synonym Full Names: | nth-like DNA glycosylase 1 |
| NCBI Official Symbol: | NTHL1�� |
| NCBI Official Synonym Symbols: | FAP3; NTH1; OCTS3; hNTH1�� |
| NCBI Protein Information: | endonuclease III-like protein 1 |
| UniProt Protein Name: | Endonuclease III-like protein 1 |
| UniProt Synonym Protein Names: | Bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase |
| Protein Family: | Neutral trehalase |
| UniProt Gene Name: | NTHL1�� |
| UniProt Entry Name: | NTH_HUMAN |
