The Human NTN1 (Netrin-1) ELISA Kit is specifically designed for the precise measurement of Netrin-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Netrin-1 is a key protein involved in axon guidance and cell migration, playing a critical role in nervous system development and regeneration.
Dysregulation of Netrin-1 has been linked to various neurological disorders, making it a valuable biomarker for studying these conditions and exploring potential treatment options.Overall, the Human NTN1 ELISA Kit provides researchers with a reliable tool to investigate the role of Netrin-1 in different pathologies and gain a deeper understanding of its therapeutic implications.
Product Name:
Human Ntn1 (Netrin 1) ELISA Kit
SKU:
HUES03203
Target:
Human Ntn1 (Netrin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Ntn1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Ntn1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Ntn1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Ntn1. You can calculate the concentration of Human Ntn1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
87-101
95-109
Average (%)
91
94
102
1:4
Range (%)
86-99
83-98
87-98
Average (%)
92
89
92
1:8
Range (%)
91-108
83-96
88-102
Average (%)
98
88
94
1:16
Range (%)
91-105
87-101
84-100
Average (%)
98
93
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
99
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
92-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.29
242.29
869.17
117.67
241.42
902.98
Standard deviation
6.12
11.39
45.11
6.46
11.85
48.04
C V (%)
5.55
4.7
5.19
5.49
4.91
5.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Ntn1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Ntn1 in samples. No significant cross-reactivity or interference between Human Ntn1 and analogues was observed.
