Human NUDT14 Recombinant Protein (RPPB5822)
- SKU:
- RPPB5822
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O95848
- Research Area:
- Enzymes
Description
| Product Name: | Human NUDT14 Recombinant Protein |
| Product Code: | RPPB5822 |
| Size: | 20µg |
| Species: | Human |
| Target: | NUDT14 |
| Synonyms: | UGPP, UGPPase, Uridine diphosphate glucose pyrophosphatase, UDPG pyrophosphatase, Nucleoside diphosphate-linked moiety X motif 14, Nudix motif 14, NUDT14. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The NUDT14 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMERIEGA SVGRCAASPY LRPLTLHYRQ NGAQKSWDFM KTHDSVTVLL FNSSRRSLVL VKQFRPAVYA GEVERRFPGS LAAVDQDGPR ELQPALPGSA GVTVELCAGL VDQPGLSLEE VACKEAWEEC GYHLAPSDLR RVATYWSGVG LTGSRQTMFY TEVTDAQRSG PGGGLVEEGE LIEVVHLPLE GAQAFADDPD IPKTLGVIFG VSWFLSQVAP NLDLQ |
Uridine diphosphate glucose pyrophosphatase (NUDT14), is a part of the nudix hydrolase family. NUDT14 is a cytoplasmic protein which contains one nudix hydrolase domain and acts as the sugar donor in numerous glycosylation reactions, including those involved in the production of glycogen. NUDT14 hydrolyzes ADP-ribose into ribose 5-phosphate and AMP, and UDP-glucose to glucose 1-phosphate and UMP. NUDT14 is a homodimer which binds magnesium as a cofactor and is encoded by a gene located on human chromosome 14.
NUDT14 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 245 amino acids (1-222) and having a molecular mass of 26.5kDa. NUDT14 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NUDT14: Hydrolyzes UDP-glucose to glucose 1-phosphate and UMP and ADP-ribose to ribose 5-phosphate and AMP. The physiological substrate is probably UDP-glucose. Poor activity on other substrates such as ADP-glucose, CDP-glucose, GDP-glucose and GDP- mannose. Belongs to the Nudix hydrolase family.Protein type: Hydrolase; EC 3.6.1.45Chromosomal Location of Human Ortholog: 14q32.33Cellular Component: cytoplasmMolecular Function: protein binding; ADP-ribose diphosphatase activity; metal ion binding; UDP-sugar diphosphatase activityBiological Process: metabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | UDP-glucose (UDPG) acts as the sugar donor in numerous glycosylation reactions, including those involved in the production of glycogen. NUDT14 is a UDPG pyrophosphatase (EC 3.6.1.45) that hydrolyzes UDPG to produce glucose 1-phosphate and UMP (Yagi et al., 2003 [PubMed 12429023]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | O95848 |
| NCBI GenInfo Identifier: | 37594467 |
| NCBI Gene ID: | 256281 |
| NCBI Accession: | NP_803877.2 |
| UniProt Secondary Accession: | O95848,Q86SJ8 |
| UniProt Related Accession: | O95848 |
| Molecular Weight: | 24,118 Da |
| NCBI Full Name: | uridine diphosphate glucose pyrophosphatase |
| NCBI Synonym Full Names: | nudix (nucleoside diphosphate linked moiety X)-type motif 14 |
| NCBI Official Symbol: | NUDT14�� |
| NCBI Official Synonym Symbols: | UGPP; UGPPase�� |
| NCBI Protein Information: | uridine diphosphate glucose pyrophosphatase; UDPG pyrophosphatase; UDP-sugar diphosphatase |
| UniProt Protein Name: | Uridine diphosphate glucose pyrophosphatase |
| UniProt Synonym Protein Names: | Nucleoside diphosphate-linked moiety X motif 14; Nudix motif 14 |
| Protein Family: | Nudix hydrolase |
| UniProt Gene Name: | NUDT14�� |
| UniProt Entry Name: | NUD14_HUMAN |
