Human OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit
The Oxidized Low-Density Lipoprotein (OxLDL) ELISA Kit is expertly crafted to quantitatively measure OxLDL levels in various human samples. OxLDL, a crucial biomarker of oxidative stress and a key player in atherosclerosis development, serves as an essential indicator of lipid peroxidation and vascular damage.
Accurate quantification of OxLDL provides valuable insights into cardiovascular health, allowing for a deeper understanding of lipid metabolism and cardiovascular risk assessment. By ensuring exceptional sensitivity and specificity, this ELISA kit delivers precise and reproducible results. Manufactured under rigorous quality control standards, the kit offers robust performance and user-friendly procedures, making it an ideal choice for both research and clinical applications. Rely on Assay Genie's OxLDL ELISA Kit for accurate and reliable quantification of this pivotal cardiovascular biomarker in your studies.
Product Name:
Human OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit
SKU:
AEES00057
Target:
Human OxLDL (Oxidized Low Density Lipoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OxLDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OxLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OxLDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OxLDL. You can calculate the concentration of Human OxLDL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-106
97-109
85-99
Average (%)
103
104
93
1:4
Range (%)
99-110
91-99
93-105
Average (%)
105
94
97
1:8
Range (%)
85-101
91-102
94-104
Average (%)
93
94
100
1:16
Range (%)
88-98
90-103
94-107
Average (%)
94
98
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-105
101
EDTA plasma (n=5)
93-105
98
Cell culture media (n=5)
97-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
171.51
483.93
1655.14
179.86
490.8
1854.26
Standard deviation
9.96
22.65
89.54
9.62
23.71
90.49
C V (%)
5.81
4.68
5.41
5.35
4.83
4.88
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human OxLDL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OxLDL in samples. No significant cross-reactivity or interference between Human OxLDL and analogues was observed.
