Human PAPPA (Pregnancy Associated Plasma Protein A) ELISA Kit
The Human PAPPA (Pregnancy-Associated Plasma Protein A) ELISA Kit is specifically designed for the precise measurement of PAPPA levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an excellent tool for various research applications.PAPPA is a vital protein involved in pregnancy-related processes, including fetal development and maternal health. Abnormal levels of PAPPA have been linked to complications during pregnancy, such as preeclampsia and fetal growth restriction. Studying PAPPA levels can provide valuable insights into these conditions and aid in the development of effective diagnostic and therapeutic strategies.Get your Human PAPPA ELISA Kit today and unlock the potential to advance your research in the fields of obstetrics, gynecology, and reproductive biology.
Product Name:
Human PAPPA (Pregnancy Associated Plasma Protein A) ELISA Kit
SKU:
HUES01420
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
