The Human Pappa2 (Pappalysin-2) CLIA Kit is a cutting-edge tool designed for the precise measurement of Pappa2 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Pappa2, also known as Pappalysin-2, is a vital enzyme involved in the regulation of insulin-like growth factor (IGF) signaling and plays a crucial role in various physiological processes such as growth and development.
Dysregulation of Pappa2 has been associated with various disorders including diabetes, cancer, and metabolic syndrome, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human Pappa2 CLIA Kit provides researchers with a reliable and efficient tool for investigating the role of Pappa2 in health and disease, offering new insights into its mechanisms and potential therapeutic targets.
Product Name:
Human PAPPA2 (Pappalysin 2) CLIA Kit
SKU:
HUES00788
Target:
Human PAPPA2 (Pappalysin 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PAPPA2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAPPA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAPPA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PAPPA2. You can calculate the concentration of Human PAPPA2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
93-105
97-114
Average (%)
102
99
104
1:4
Range (%)
99-112
90-103
90-104
Average (%)
105
97
97
1:8
Range (%)
99-116
102-118
84-100
Average (%)
106
109
91
1:16
Range (%)
88-103
93-109
91-103
Average (%)
95
101
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
97-109
102
Cell culture media (n=5)
99-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
193.1
488.02
1923.88
175.91
451.09
2052.58
Standard deviation
19.52
37.04
190.46
14.67
42.99
189.04
C V (%)
10.11
7.59
9.9
8.34
9.53
9.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PAPPA2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PAPPA2 in samples. No significant cross-reactivity or interference between Human PAPPA2 and analogues was observed.
