Human PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) CLIA Kit
The Human PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) CLIA Kit is a powerful tool for accurately measuring PCSK9 levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers dependable and consistent results, making it perfect for a wide range of research purposes.PCSK9 is a key player in regulating cholesterol levels by binding to and degrading the LDL receptor, ultimately affecting cholesterol metabolism.
Dysregulation of PCSK9 is associated with cardiovascular diseases such as hypercholesterolemia, making it a valuable target for therapeutic interventions.Order the Human PCSK9 CLIA Kit today from Assay Genie and enhance your research on cholesterol metabolism, cardiovascular health, and potential treatment strategies.
Product Name:
Human PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) CLIA Kit
SKU:
HUES00896
Target:
Human PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PCSK9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PCSK9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCSK9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PCSK9. You can calculate the concentration of Human PCSK9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
91-105
98-111
Average (%)
100
98
105
1:4
Range (%)
85-97
98-114
95-107
Average (%)
90
104
101
1:8
Range (%)
87-98
89-102
90-108
Average (%)
92
96
98
1:16
Range (%)
97-111
95-106
89-101
Average (%)
103
101
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
100
EDTA plasma (n=5)
98-115
105
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
397.37
862.26
3102.37
384.1
803.11
2861.68
Standard deviation
44.55
73.46
195.14
44.06
68.02
231.51
C V (%)
11.21
8.52
6.29
11.47
8.47
8.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PCSK9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PCSK9 in samples. No significant cross-reactivity or interference between Human PCSK9 and analogues was observed.
