Human PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) ELISA Kit
The Assay Genie Human PCSK9 ELISA Kit is meticulously crafted for the precise and quantitative determination of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) levels in a variety of biological samples. PCSK9 is a critical enzyme known for its role in regulating low-density lipoprotein receptor (LDL-R) levels, thereby impacting cholesterol metabolism and cardiovascular health. Accurate measurement of PCSK9 is essential in understanding its involvement in lipid metabolism, cardiovascular diseases, and potential therapeutic interventions. Our PCSK9 ELISA Kit is characterized by exceptional sensitivity and specificity, ensuring accurate and reproducible results in your research endeavors.
Manufactured under strict quality control standards, this kit delivers robust performance and ease of use, making it a top choice for investigations in lipid biology, cardiovascular research, and related fields. Trust Assay Genie's PCSK9 ELISA Kit for reliable and high-quality quantification of PCSK9, empowering your scientific studies with precise data and insights.
Product Name:
Human PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) ELISA Kit
SKU:
AEES00182
Target:
Human PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PCSK9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PCSK9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCSK9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PCSK9. You can calculate the concentration of Human PCSK9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
92-109
85-99
Average (%)
102
99
90
1:4
Range (%)
92-103
93-108
84-95
Average (%)
98
99
89
1:8
Range (%)
98-114
92-107
85-97
Average (%)
104
100
91
1:16
Range (%)
97-113
89-104
87-100
Average (%)
103
96
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
93
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
84-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
271.19
478.9
2365.31
268.21
510.89
2242.14
Standard deviation
18.01
25.48
109.04
17.57
30.55
103.14
C V (%)
6.64
5.32
4.61
6.55
5.98
4.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PCSK9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PCSK9 in samples. No significant cross-reactivity or interference between Human PCSK9 and analogues was observed.
