Human PDCD1LG1 (Programmed Cell Death Protein 1 Ligand 1) ELISA Kit
The Human PDCD1LG1 (Programmed Cell Death Protein 1 Ligand 1) ELISA Kit is specifically designed for the precise measurement of PDCD1LG1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an invaluable tool for various research applications.PDCD1LG1 is a critical protein involved in immune responses, particularly in regulating T-cell activation and promoting T-cell apoptosis. Dysregulation of PDCD1LG1 has been linked to autoimmune diseases, cancer, and other immune-related disorders, highlighting its importance as a potential biomarker for understanding and developing treatments for these conditions.This ELISA kit provides researchers with a reliable and efficient method for studying the role of PDCD1LG1 in various physiological and pathological processes, ultimately advancing our understanding of immune regulation and disease progression.
Product Name:
Human PDCD1LG1 (Programmed Cell Death Protein 1 Ligand 1) ELISA Kit
SKU:
HUES02561
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
