The Human PDCN (Podocin) CLIA Kit is a cutting-edge diagnostic tool specifically designed for the precise measurement of podocin levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it an invaluable asset for various research endeavors.Podocin plays a critical role in maintaining the structural integrity of the kidney's filtration barrier, making it a key player in renal health and disease.
Abnormal podocin levels have been linked to various kidney disorders, including glomerular diseases and proteinuria. Therefore, this kit serves as a vital tool for studying these conditions and potentially developing targeted therapies to address them.Overall, the Human PDCN (Podocin) CLIA Kit offers researchers a reliable and efficient method for quantifying podocin levels, leading to a deeper understanding of kidney function and related pathologies.
Product Name:
Human PDCN (Podocin) CLIA Kit
SKU:
HUES00543
Target:
Human PDCN (Podocin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PDCN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDCN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDCN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PDCN. You can calculate the concentration of Human PDCN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-101
86-99
94-109
Average (%)
92
93
102
1:4
Range (%)
88-102
101-115
88-99
Average (%)
94
108
94
1:8
Range (%)
102-115
100-114
87-98
Average (%)
109
107
92
1:16
Range (%)
89-103
85-100
89-105
Average (%)
96
92
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
91
EDTA plasma (n=5)
85-96
91
Cell culture media (n=5)
96-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
209.88
624.25
1520.46
216.53
679.39
1658.18
Standard deviation
25.56
48.19
170.75
27.78
77.59
183.73
C V (%)
12.18
7.72
11.23
12.83
11.42
11.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PDCN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PDCN in samples. No significant cross-reactivity or interference between Human PDCN and analogues was observed.
